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Biochemistry and molecular biology of fishes. vol 3 - analytical techniques / compil. P. Hochachka ; co-aut. T. Mommsen Nível de parte analítica: Buffered salt solutions, culture media and tissue homogenization buffers • Analyzing phosphatidylinositol cycling and protein kinase c involvement • Ca2+ transport across plasma membranes • Metabolite transport in fish red blood cells and hepatocytes • Analysis of enzyme regulation via reversible phosphorylation and enzyme binding interactions with macromolecules • In vivo nuclear magnetic resonance spectroscopy of aquatic animals • Bolus - injection of radiolabels in the study of steady state glucose metabolism • Developing an elisa for a model protein - vitellogenin • Hormone measurement by elisa : application to fish gonadotrophin • Radioimmunoassay of fish growth hormone, prolactin, and somatolactin • Development of radiommunoassay for a model peptide hormone : insulin • Incorporation of 35 s-sulfate into branchial cartilage : a biological model to study hormonal regulation of skeletal ... • Use of membrane vesicle techniques to characterize nutrient transport processes of the teleost gastrointestinal tract • Peroxisomes and peroxisomal b-oxidation • Fish mitochondria • Membrane preparation and receptor assay - adenosine • Membrane, cell preparation and receptor assay - catecholamines • Steroid receptors in fish : membrane and intracellular preparations • Thyroid hormone : deiodinase and receptor assays • Peptide receptor assays : insulin receptor • Xenobiotics : designing an in vitro system to study enzymes and metabolism • Measurement of intracellular calcium in fish liver cells • Measurement of intracellular ph in fish hepatocytes • Perifusion of hepatocytes • Organ culture of fish tissues • Hepatocytes : isolation, maintenance and utilization • In vitro culture of fishleukocytes • Isolation and culture of head kidney macrophages • Enzymes of lipogenesis • Adipose tissue • Oogenesis • Isolated cardiac myocytes • Primary culture of rainbow trout corpuscles of stannius • Tests cells : isolation and culture • Isolation of gill cells • Opercular membranes and skin • Photophores : the analysis of bioluminescent systems • Teleost pituitary cells : isolation, culture and use • Isolated rete • Perifusion of pancreatic endocrine tissue of teleost fish • Perifusion of pancreatic islets • Extracorporeal circulation • Cell lines : availability, propagation and isolation • Methods for assessing kidney and urinary bladder function in fish • Isolated perfused fish hearts • Intact and demembranated muscle fibres • Techniques for recording from electric organs • Injection techniques • Hepatectomy • Microsurgery : gonadectomy • Surgical extirpation of the stannius corpuscles : stanniectomy • Hypophysectomy of fish • Surgery : selective isle removal and total pancreatectomy • Cannulation techniques • AnaestheticsPublicação: Amsterdam : Elsevier, 1994Descrição: 700 p.Disponibilidade:
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